Influence of nitrogen water interaction on leaf functional traits of dominant species in warm temperate forest

Abstract

Plant functional traits are indicative of plant responses to environmental changes, influencing ecosystem functions. Leaves, as a key focus in studying plant functional traits, present an area where the impact of nitrogen deposition and altered rainfall patterns on functional diversity remains ambiguous. To elucidate plant response mechanisms to environmental factors, we employed a canopy-based platform to add nitrogen, water, and their combination. We assessed the functional traits and community-weighted mean of the leaves of three dominant trees and three dominant shrubs. The results showed that nitrogen addition to the canopy significantly increased the leaf dry matter content of the Celtis sinensis Pers, but markedly decreased the specific leaf area of the Liquidambar formosana Hance. The nitrogen-water interaction did not notably affect the specific leaf area and equivalent water thickness of leaves. Canopy addition of nitrogen, water, and their combined interaction substantially lowered leaf nitrogen content and markedly increased leaf C/N. The structural equation model demonstrated a significant negative correlation between leaf dry matter content, equivalent water thickness, and leaf nitrogen content, as well as between equivalent water thickness and leaf phosphorus content. Our results provide evidence for the adaptation of plants to the environment and different strategies for resource and energy utilization.

Keywords

• canopy nitrogen addition
• canopy water addition
• nitrogen water interaction
• leaf functional traits
• community weighted mean