Links between Disease Severity, Bacterial Infections and Oxidative Stress in Cystic Fibrosis

Abstract

Cystic fibrosis (CF) is one of the most common, yet fatal genetic diseases in Caucasians. The presence of a defective CF transmembrane conductance regulator and the massive neutrophils influx into the airways contribute to an imbalance in epithelial cell processes and extracellular fluids and lead to excessive production of reactive oxygen species and intensification of oxidative stress. The study included 16 controls and 42 participants with CF aged 10 to 38. The products of protein oxidation, total antioxidant capacity (TAC) and markers of lipid peroxidation were estimated in the serum of the subjects. Furthermore, we compared the level of oxidative stress in patients with CF according to the severity of disease and type of bacterial infection. Thiol groups and serum TAC decreased significantly in patients with CF (p p Pseudomonas aeruginosa had elevated 3-nitrotyrosine concentration levels (p Staphylococcus aureus noted a decrease in thiol groups (p P. aeruginosa or S. aureus had a slight effect on oxidative stress, while co-infection by two species did not affect the level of oxidative stress.

Keywords

• bacterial infection
• disease severity
• protein oxidation
• lipid peroxidation
• total antioxidant capacity