100 Hz ROCS microscopy correlated with fluorescence reveals cellular dynamics on different spatiotemporal scales

Felix Jünger; Dominic Ruh; Dominik Strobel; Rebecca Michiels; Dominik Huber; Annette Brandel; Josef Madl; Alina Gavrilov; Michael Mihlan; Caterina Cora Daller; Eva A. Rog-Zielinska; Winfried Römer; Tim Lämmermann; Alexander Rohrbach

doi:10.1038/s41467-022-29091-0

Nature Communications (Apr 2022)

100 Hz ROCS microscopy correlated with fluorescence reveals cellular dynamics on different spatiotemporal scales

Felix Jünger,
Dominic Ruh,
Dominik Strobel,
Rebecca Michiels,
Dominik Huber,
Annette Brandel,
Josef Madl,
Alina Gavrilov,
Michael Mihlan,
Caterina Cora Daller,
Eva A. Rog-Zielinska,
Winfried Römer,
Tim Lämmermann,
Alexander Rohrbach

Affiliations

Felix Jünger: Laboratory for Bio- and Nano-Photonics, Department of Microsystems Engineering - IMTEK, University of Freiburg
Dominic Ruh: Laboratory for Bio- and Nano-Photonics, Department of Microsystems Engineering - IMTEK, University of Freiburg
Dominik Strobel: Laboratory for Bio- and Nano-Photonics, Department of Microsystems Engineering - IMTEK, University of Freiburg
Rebecca Michiels: Laboratory for Bio- and Nano-Photonics, Department of Microsystems Engineering - IMTEK, University of Freiburg
Dominik Huber: Laboratory for Bio- and Nano-Photonics, Department of Microsystems Engineering - IMTEK, University of Freiburg
Annette Brandel: Faculty of Biology, University of Freiburg
Josef Madl: Signalling Research Centres BIOSS and CIBSS, University of Freiburg
Alina Gavrilov: Max Planck Institute of Immunobiology and Epigenetics
Michael Mihlan: Max Planck Institute of Immunobiology and Epigenetics
Caterina Cora Daller: Institute for Experimental Cardiovascular Medicine, University Heart Center Freiburg - Bad Krozingen, and Faculty of Medicine, University of Freiburg
Eva A. Rog-Zielinska: Institute for Experimental Cardiovascular Medicine, University Heart Center Freiburg - Bad Krozingen, and Faculty of Medicine, University of Freiburg
Winfried Römer: Faculty of Biology, University of Freiburg
Tim Lämmermann: Max Planck Institute of Immunobiology and Epigenetics
Alexander Rohrbach: Laboratory for Bio- and Nano-Photonics, Department of Microsystems Engineering - IMTEK, University of Freiburg

DOI: https://doi.org/10.1038/s41467-022-29091-0
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 17

Abstract

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In live-cell microscopy, motion blur limits resolution and contrast. Here the authors use 100 Hz super-resolving Rotating Coherent Scattering (ROCS) microscopy on various dynamic biological systems, and time-window analysis to understand biological effects.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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