Química Nova (Jan 2013)

Biochemical properties of Bacillus sp. ITP-001 lipase immobilized with a sol gel process

  • Nayara Bezerra Carvalho,
  • José Murillo P. Barbosa,
  • Maria Vanessa S. Oliveira,
  • Alini T. Fricks,
  • Álvaro S. Lima,
  • Cleide Mara F. Soares

Journal volume & issue
Vol. 36, no. 1
pp. 52 – 58

Abstract

Read online

This work presents biochemical characterization of a lipase from a new strain of Bacillus sp. ITP-001, immobilized using a sol gel process (IB). The results from the biochemical characterization of IB showed increased activity for hydrolysis, with 526.63 U g-1 at pH 5.0 and 80 ºC, and thermal stability at 37 ºC. Enzymatic activity was stimulated by ions such as EDTA, Fe+3, Mn+2, Zn+2, and Ca+2, and in various organic solvents. Kinetic parameters obtained for the IB were Km = 14.62 mM, and Vmax = 0.102 mM min-1 g-1. The results of biochemical characterization revealed the improved catalytic properties of IB.

Keywords