Investigation of Serum Paraoxonase 1 (PON1) Activities in Postprandial Lipemia

Abstract

Aim: Paraoxonase-1 (PON1) whic is present in HDL structure, is an enzyme that decreases the oxidative stress in atherosclerotic lesions by preventing the HDL and LDL against to ox-idation. Thu study aims to determine the paraoxonase, arylesterase and lactonease activities of PON1 enzyme, taking into account the response of healthy subjects to the oral triglyceride tolerance test (OTTT). Material and Method: Study group included 96 healthy subjects (45 female and 51 male with age range of 18-55 years). Study group was divided into three groups according to the area under curve (AUC) values calculated by using triglyceride levels at the fasting state and at 2nd, 4th and 6th hours after the high fat diet (OTTT). PON1 enzyme activity levels were determined by spectrofotometric methods. PON1 enzyme activities and other parameters in lower OTTT response group were compared to that of the upper group. Results: Atherogenic lipid profile, increased total cholesterol and LDL-C and decreased HDL-C levels, was observed in subjects with the upper group men when compared to the lower group. PON1 lactonase activity was significantly lower in men than that of women (P<0.05). On the other hand, PON1 lactonase activity showed time-depended increase during OTTT in both sex. PON1 arylesterase activity in subjects with the upper group was significantly higher than that of the lower groups (P<0.022) in women. Conclusion: It was observed that the upper groups with high OTTT response had an atherogenic lipid profile. It has been thought that PON1 enzyme activities tend to increase in the Postprandial period as a response to oxidative stress and detailed studies are needed.

Keywords

• arilesteraz
• dislipidemi
• laktonaz
• paraoksonaz
• yüksek yağlı diyet
• arilesterase
• dyslipidemia
• high fat diet
• lactonase
• paraoxonase