Cellular Immune Status Indicators of Anti-Plague Vaccinated Persons, Living on the Caspian Sandy Natural Plague Focus Territory

Abstract

Aim. To compare by flow cytometry the cellular immune status indicators in persons, living on the territory of the Caspian sandy natural plague focus for epidemic indications. Materials and methods. Whole blood leukocytes of 120 people were examined by flow cytometry with using the two four-color labeled monoclonal antibody reagents (Cyto-Stat CD45-FITC, CD4-PE, CD8-ECD, CD3-PC5 и Cyto-Stat CD45-FITC, CD56-PE, CD19-ECD, CD3-PC5) and by means of CD95-FITC reagent. The results were taken into account before and in a month and 6 months after vaccination. We were determined in the blood the relative content of lymphocytes, monocytes, granulocytes, leukocytes carrying an early apoptosis marker (CD95), T and B- lymphocytes, NK cells as well T – lymphocyte helpers (Th) and cytotoxic T-lymphocytes (Tcyt) for immunoregulatory index (IRI) calculation. Results. Before anti-plague vaccination in 14 people (11,7%) were found abnormally low immunoregulatory index values characterized the immunodeficiency state (IRI <1) and noted less intensive and less prolonged immune system cell reaction in response to anti-plague vaccination. In the other surveyed persons IRI were registered before vaccination in the range 1,4-1,5 with a smooth increase in it a month after vaccination and with preservation of the established IRI interval in a range 1,6-1,8 later 6 months after an inoculation. Conclusion. To estimate the effectiveness of anti plague vaccination within the framework of the ongoing measures quality monitoring to ensure sanitary and epidemiological welfare of the population, living on territory of the natural plague foci, the timely immune status monitoring is important in persons at risk for plague infection (hunters, shepherds, agricultural workers, medical workers).

Keywords

• natural focus
• vaccination
• plague
• immune status
• imminoregulatory index
• flow cytometry