Analiza chemiczna i spektrofotometryczna oczyszczonego i wydzielonego RNA z kiełków jeczmienia (Hordeum sativum) metodą fenolową i chloroformową [Chemical and spectrophotometric analysis of purified RNA isolated the chloroform and phenol methods from barley germs (Hordeum sativum)]

Abstract

Two different ribonucleic acids were isolated from barley germs by use of the phenol (RNA I) and chloroform method (RNA II). After separation on Sephadex G-200 RNA I was found to be homogenous, whereas RNA II showed 5 components. Furthermore, both preparations differed in chemical composition and spectrophotometric specificity.