Sanitary status of Pome and Stone Fruit Collection in Gene Bank in Republic of Srpska

Abstract

Detection of viruses presence were carried out for the 225 a of pome and stone fruit trees from the collection of the Genetic Resources Institute of University of Banja Luka, located within the Botanical Garden of the University, tested by DAS-ELISA. The pome fruit trees were analyzed on presence of the following viruses: Apple Chlorotic Leaf Spot Virus (ACLSV), Apple Stem Grooving Virus (ASGV), Apple Stem Pitting Virus (ASPV) and Apple Mosaic Virus (ApMV). The stone fruits were analyzed on presence of Plum Pox Virus (PPV), Prune Dwarf Virus (PDV) and Prunus Necrotic Ring Spot Virus (PNRSV). All samples were tested serologically by DAS-ELISA. In addition to this, virus negative pear and apple accessions were tested for 'Candidatus Phytoplasma mali' and 'Candidatus Phytoplasma pyri' presence using nested-PCR/RFLP analyses.

Keywords

• pome and stone fruit viruses
• pome fruit phytoplasmas
• das-elisa
• nested-pcr/rflp