Nature Communications (May 2019)

Atomic resolution cryo-EM structure of a native-like CENP-A nucleosome aided by an antibody fragment

  • Bing-Rui Zhou,
  • K. N. Sathish Yadav,
  • Mario Borgnia,
  • Jingjun Hong,
  • Baohua Cao,
  • Ada L. Olins,
  • Donald E. Olins,
  • Yawen Bai,
  • Ping Zhang

DOI
https://doi.org/10.1038/s41467-019-10247-4
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 7

Abstract

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CENP-A histone variants replace histones H3 at centromeres. Here the authors use a single-chain antibody fragment (scFv) to stabilize human CENP-A nucleosome containing a native α-satellite DNA and solved its structure by cryo-EM to 2.6 Å resolution, providing insight into the structure and function of the CENP-A nucleosome.