Nature Communications (May 2019)
Atomic resolution cryo-EM structure of a native-like CENP-A nucleosome aided by an antibody fragment
Abstract
CENP-A histone variants replace histones H3 at centromeres. Here the authors use a single-chain antibody fragment (scFv) to stabilize human CENP-A nucleosome containing a native α-satellite DNA and solved its structure by cryo-EM to 2.6 Å resolution, providing insight into the structure and function of the CENP-A nucleosome.