Distribution of Iron Oxide Core-Titanium Dioxide Shell Nanoparticles in VX2 Tumor Bearing Rabbits Introduced by Two Different Delivery Modalities

Tamer Refaat; Derek West; Samar El Achy; Vamsi Parimi; Jasmine May; Lun Xin; Kathleen R. Harris; William Liu; Michael Beau Wanzer; Lydia Finney; Evan Maxey; Stefan Vogt; Reed A. Omary; Daniele Procissi; Andrew C. Larson; Tatjana Paunesku; Gayle E. Woloschak

doi:10.3390/nano6080143

Nanomaterials (Aug 2016)

Distribution of Iron Oxide Core-Titanium Dioxide Shell Nanoparticles in VX2 Tumor Bearing Rabbits Introduced by Two Different Delivery Modalities

Tamer Refaat,
Derek West,
Samar El Achy,
Vamsi Parimi,
Jasmine May,
Lun Xin,
Kathleen R. Harris,
William Liu,
Michael Beau Wanzer,
Lydia Finney,
Evan Maxey,
Stefan Vogt,
Reed A. Omary,
Daniele Procissi,
Andrew C. Larson,
Tatjana Paunesku,
Gayle E. Woloschak

Affiliations

Tamer Refaat: Radiation Oncology Department, Radiology Department and Pathology Core Facility, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
Derek West: Radiation Oncology Department, Radiology Department and Pathology Core Facility, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
Samar El Achy: Clinical Oncology Department and Pathology Department, Faculty of Medicine, Alexandria University, Alexandria 21131, Egypt
Vamsi Parimi: Radiation Oncology Department, Radiology Department and Pathology Core Facility, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
Jasmine May: Radiation Oncology Department, Radiology Department and Pathology Core Facility, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
Lun Xin: Radiation Oncology Department, Radiology Department and Pathology Core Facility, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
Kathleen R. Harris: Radiation Oncology Department, Radiology Department and Pathology Core Facility, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
William Liu: Radiation Oncology Department, Radiology Department and Pathology Core Facility, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
Michael Beau Wanzer: Radiation Oncology Department, Radiology Department and Pathology Core Facility, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
Lydia Finney: X-ray Sciences Division, Advanced Photon Source, Argonne National Laboratory, Argonne, IL 60439, USA
Evan Maxey: X-ray Sciences Division, Advanced Photon Source, Argonne National Laboratory, Argonne, IL 60439, USA
Stefan Vogt: X-ray Sciences Division, Advanced Photon Source, Argonne National Laboratory, Argonne, IL 60439, USA
Reed A. Omary: Department of Radiology and Radiological Sciences, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
Daniele Procissi: Radiation Oncology Department, Radiology Department and Pathology Core Facility, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
Andrew C. Larson: Radiation Oncology Department, Radiology Department and Pathology Core Facility, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
Tatjana Paunesku: Radiation Oncology Department, Radiology Department and Pathology Core Facility, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
Gayle E. Woloschak: Radiation Oncology Department, Radiology Department and Pathology Core Facility, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA

DOI: https://doi.org/10.3390/nano6080143
Journal volume & issue: Vol. 6, no. 8
p. 143

Abstract

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This work compares intravenous (IV) versus fluoroscopy-guided transarterial intra-catheter (IC) delivery of iron oxide core-titanium dioxide shell nanoparticles (NPs) in vivo in VX2 model of liver cancer in rabbits. NPs coated with glucose and decorated with a peptide sequence from cortactin were administered to animals with developed VX2 liver cancer. Two hours after NPs delivery tumors, normal liver, kidney, lung and spleen tissues were harvested and used for a series on histological and elemental analysis tests. Quantification of NPs in tissues was done both by bulk inductively coupled plasma mass spectrometry (ICP-MS) analysis and by hard X-ray fluorescence microscopy. Both IV and IC NPs injection are feasible modalities for delivering NPs to VX2 liver tumors with comparable tumor accumulation. It is possible that this is an outcome of the fact that VX2 tumors are highly vascularized and hemorrhagic, and therefore enhanced permeability and retention (EPR) plays the most significant role in accumulation of nanoparticles in tumor tissue. It is, however, interesting to note that IV delivery led to increased sequestration of NPs by spleen and normal liver tissue, while IC delivery lead to more NP positive Kupffer cells. This difference is most likely a direct outcome of blood flow dynamics. Armed with this knowledge about nanoparticle delivery, we plan to test them as radiosensitizers in the future.

Published in Nanomaterials

ISSN: 2079-4991 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry
Website: https://www.mdpi.com/journal/nanomaterials

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