Frontiers in Microbiology (Jun 2021)
A Homeodomain-Containing Transcriptional Factor PoHtf1 Regulated the Development and Cellulase Expression in Penicillium oxalicum
- Hao Guo,
- Hao Guo,
- Hao Guo,
- Gen Xu,
- Gen Xu,
- Gen Xu,
- Ruimei Wu,
- Zhigang Li,
- Zhigang Li,
- Zhigang Li,
- Mengdi Yan,
- Mengdi Yan,
- Mengdi Yan,
- Zhilei Jia,
- Zhilei Jia,
- Zhilei Jia,
- Zhonghai Li,
- Zhonghai Li,
- Zhonghai Li,
- Mei Chen,
- Mei Chen,
- Mei Chen,
- Xiaoming Bao,
- Xiaoming Bao,
- Xiaoming Bao,
- Yinbo Qu
Affiliations
- Hao Guo
- State Key Laboratory of Biobased Material and Green Papermaking, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China
- Hao Guo
- School of Bioengineering, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China
- Hao Guo
- Shandong Provincial Key Laboratory of Microbial Engineering, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China
- Gen Xu
- State Key Laboratory of Biobased Material and Green Papermaking, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China
- Gen Xu
- School of Bioengineering, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China
- Gen Xu
- Shandong Provincial Key Laboratory of Microbial Engineering, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China
- Ruimei Wu
- Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, China
- Zhigang Li
- State Key Laboratory of Biobased Material and Green Papermaking, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China
- Zhigang Li
- School of Bioengineering, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China
- Zhigang Li
- Shandong Provincial Key Laboratory of Microbial Engineering, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China
- Mengdi Yan
- State Key Laboratory of Biobased Material and Green Papermaking, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China
- Mengdi Yan
- School of Bioengineering, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China
- Mengdi Yan
- Shandong Provincial Key Laboratory of Microbial Engineering, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China
- Zhilei Jia
- State Key Laboratory of Biobased Material and Green Papermaking, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China
- Zhilei Jia
- School of Bioengineering, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China
- Zhilei Jia
- Shandong Provincial Key Laboratory of Microbial Engineering, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China
- Zhonghai Li
- State Key Laboratory of Biobased Material and Green Papermaking, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China
- Zhonghai Li
- School of Bioengineering, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China
- Zhonghai Li
- Shandong Provincial Key Laboratory of Microbial Engineering, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China
- Mei Chen
- State Key Laboratory of Biobased Material and Green Papermaking, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China
- Mei Chen
- School of Bioengineering, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China
- Mei Chen
- Shandong Provincial Key Laboratory of Microbial Engineering, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China
- Xiaoming Bao
- State Key Laboratory of Biobased Material and Green Papermaking, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China
- Xiaoming Bao
- School of Bioengineering, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China
- Xiaoming Bao
- Shandong Provincial Key Laboratory of Microbial Engineering, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China
- Yinbo Qu
- State Key Laboratory of Microbial Technology, School of Life Sciences, National Glycoengineering Research Center, Shandong University, Qingdao, China
- DOI
- https://doi.org/10.3389/fmicb.2021.671089
- Journal volume & issue
-
Vol. 12
Abstract
Homeodomain-containing transcription factors (Htfs) play important roles in animals, fungi, and plants during some developmental processes. Here, a homeodomain-containing transcription factor PoHtf1 was functionally characterized in the cellulase-producing fungi Penicillium oxalicum 114-2. PoHtf1 was shown to participate in colony growth and conidiation through regulating the expression of its downstream transcription factor BrlA, the key regulator of conidiation in P. oxalicum 114-2. Additionally, PoHtf1 inhibited the expression of the major cellulase genes by coordinated regulation of cellulolytic regulators CreA, AmyR, ClrB, and XlnR. Furthermore, transcriptome analysis showed that PoHtf1 participated in the secondary metabolism including the pathway synthesizing conidial yellow pigment. These data show that PoHtf1 mediates the complex transcriptional-regulatory network cascade between developmental processes and cellulolytic gene expression in P. oxalicum 114-2. Our results should assist the development of strategies for the metabolic engineering of mutants for applications in the enzymatic hydrolysis for biochemical production.
Keywords
