Biotecnología Vegetal (Jan 2007)

Effect of explant density and culture medium volume on <em>in vitro</em> propagation of ‘FHIA-21’ (AAAB) hybrid in Temporary Immersion System

  • Milagros Basail Pérez,
  • Rafael Gómez-Kosky,
  • Victor Medero Vega,
  • Eneida Otero Gálvez,
  • Marlenys Torres Delgado,
  • Manuel Cabrera Jova,
  • Jorge López Torres,
  • Magaly García García,
  • Arletys Santos Pino,
  • Aymé Rayas Cabrera,
  • José de la C. Ventura Martín,
  • Maricel Bauta Toledo,
  • Miguel Álvarez Mesa,
  • Eriker Páz Chávez,
  • Yoel Beovidez García,
  • Julia Albert Llerena,
  • Alexis Ortega Ortiz,
  • Alberto Espinosa Cuéllar,
  • Jesús García Ruiz

Journal volume & issue
Vol. 7, no. 1

Abstract

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The need of producing high quality planting material has required the searching of new alternatives to increase the efficiency of in vitro propagation methods and their automation, such as Temporary Immersion Systems. This work has been carried out to increase the multiplication coefficient in mass propagation of hybrid ‘FHIA-21’ (AAAB) in Temporary Immersion Systems. Different culture medium volumes per explant and densities of planting materials per unit were studied at the same immersion frequency. The highest multiplication coefficient rate, (14.99 and 16.02 respectively), was obtained when 40 ml culture medium volume were used at a density of 70 explants/flask. The use of Temporary Immersion System allowed increasing the multiplication coefficient in hybrid ‘FHIA-21’ (AAAB) and the highest quality multiplication coefficient for rooting stage and further acclimatization in field conditions. Key words: hybrid, liquid culture medium, multiplication coefficient