Chromatographic analysis of immobilized cefotaxime

Abstract

The aim of the present work was to widen the application of an in-house developed fast, flexible and sensitive high performance liquid chromatography (HPLC) method to the assessment of cefotaxime sodium from aqueous samples. The method was applied to establish the release profile of cefotaxime sodium immobilised in MCM-41 nanoparticles using pH controlled release in aqueous medium. The analytical method proved to be sensible, repeatable (RSD < 1.5 %) and reproducible (RSD < 1 %) in the concentration range studied (0.01-10 μg•mL-1), limit of detection and limit of quantification were 0.036 and 0.12 μg•mL-1 respectively, suitable for the analysis of release of a single active ingredient, having a short analysis time (10 minutes).

Keywords

• cefotaxime sodium
• cephalosporins
• controlled release
• HPLC