Thermodynamics and kinetics guided probe design for uniformly sensitive and specific DNA hybridization without optimization

Xin Chen; Na Liu; Liquan Liu; Wei Chen; Na Chen; Meng Lin; Jiaju Xu; Xing Zhou; Hongbo Wang; Meiping Zhao; Xianjin Xiao

doi:10.1038/s41467-019-12593-9

Nature Communications (Oct 2019)

Thermodynamics and kinetics guided probe design for uniformly sensitive and specific DNA hybridization without optimization

Xin Chen,
Na Liu,
Liquan Liu,
Wei Chen,
Na Chen,
Meng Lin,
Jiaju Xu,
Xing Zhou,
Hongbo Wang,
Meiping Zhao,
Xianjin Xiao

Affiliations

Xin Chen: Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology
Na Liu: Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology
Liquan Liu: Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology
Wei Chen: Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University
Na Chen: Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology
Meng Lin: Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology
Jiaju Xu: Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology
Xing Zhou: Department of Obstetrics and Gynaecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
Hongbo Wang: Department of Obstetrics and Gynaecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
Meiping Zhao: Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University
Xianjin Xiao: Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology

DOI: https://doi.org/10.1038/s41467-019-12593-9
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 12

Abstract

Read online

Optimisation of nucleic acid probes and blocker strands can be laborious. Here the authors construct a theoretical model of competitive DNA hybridisation to design DNA probes for optimisation-free mutation detection.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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