Structures and transport dynamics of a Campylobacter jejuni multidrug efflux pump

Chih-Chia Su; Linxiang Yin; Nitin Kumar; Lei Dai; Abhijith Radhakrishnan; Jani Reddy Bolla; Hsiang-Ting Lei; Tsung-Han Chou; Jared A. Delmar; Kanagalaghatta R. Rajashankar; Qijing Zhang; Yeon-Kyun Shin; Edward W. Yu

doi:10.1038/s41467-017-00217-z

Nature Communications (Aug 2017)

Structures and transport dynamics of a Campylobacter jejuni multidrug efflux pump

Chih-Chia Su,
Linxiang Yin,
Nitin Kumar,
Lei Dai,
Abhijith Radhakrishnan,
Jani Reddy Bolla,
Hsiang-Ting Lei,
Tsung-Han Chou,
Jared A. Delmar,
Kanagalaghatta R. Rajashankar,
Qijing Zhang,
Yeon-Kyun Shin,
Edward W. Yu

Affiliations

Chih-Chia Su: Department of Physics and Astronomy, Iowa State University
Linxiang Yin: Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University
Nitin Kumar: Department of Chemistry, Iowa State University
Lei Dai: Department of Veterinary Microbiology, College of Veterinary Medicine, Iowa State University
Abhijith Radhakrishnan: Department of Chemistry, Iowa State University
Jani Reddy Bolla: Department of Chemistry, Iowa State University
Hsiang-Ting Lei: Department of Chemistry, Iowa State University
Tsung-Han Chou: Department of Physics and Astronomy, Iowa State University
Jared A. Delmar: Department of Physics and Astronomy, Iowa State University
Kanagalaghatta R. Rajashankar: NE-CAT and Department of Chemistry and Chemical Biology, Cornell University, Argonne National Laboratory
Qijing Zhang: Department of Veterinary Microbiology, College of Veterinary Medicine, Iowa State University
Yeon-Kyun Shin: Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University
Edward W. Yu: Department of Physics and Astronomy, Iowa State University

DOI: https://doi.org/10.1038/s41467-017-00217-z
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 11

Abstract

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Multidrug efflux pumps significantly contribute for bacteria resistance to antibiotics. Here the authors present the structure of Campylobacter jejuni CmeB pump combined with functional FRET assays to propose a transport mechanism where each CmeB protomers is functionally independent from the trimer.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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