Nature Communications (Dec 2017)
Efficient transgenesis and annotated genome sequence of the regenerative flatworm model Macrostomum lignano
- Jakub Wudarski,
- Daniil Simanov,
- Kirill Ustyantsev,
- Katrien de Mulder,
- Margriet Grelling,
- Magda Grudniewska,
- Frank Beltman,
- Lisa Glazenburg,
- Turan Demircan,
- Julia Wunderer,
- Weihong Qi,
- Dita B. Vizoso,
- Philipp M. Weissert,
- Daniel Olivieri,
- Stijn Mouton,
- Victor Guryev,
- Aziz Aboobaker,
- Lukas Schärer,
- Peter Ladurner,
- Eugene Berezikov
Affiliations
- Jakub Wudarski
- European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1
- Daniil Simanov
- European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1
- Kirill Ustyantsev
- Institute of Cytology and Genetics, Prospekt Lavrentyeva 10
- Katrien de Mulder
- Hubrecht Institute-KNAW and University Medical Centre Utrecht, Uppsalalaan 8
- Margriet Grelling
- European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1
- Magda Grudniewska
- European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1
- Frank Beltman
- European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1
- Lisa Glazenburg
- European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1
- Turan Demircan
- Hubrecht Institute-KNAW and University Medical Centre Utrecht, Uppsalalaan 8
- Julia Wunderer
- Institute of Zoology and Center for Molecular Biosciences Innsbruck, University of Innsbruck, Technikerstr. 25
- Weihong Qi
- Functional Genomics Center Zurich, Winterthurerstrasse 190
- Dita B. Vizoso
- Evolutionary Biology, Zoological Institute, University of Basel, Vesalgasse 1
- Philipp M. Weissert
- European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1
- Daniel Olivieri
- European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1
- Stijn Mouton
- European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1
- Victor Guryev
- European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1
- Aziz Aboobaker
- Department of Zoology, University of Oxford, Tinbergen Building, South Parks Road
- Lukas Schärer
- Evolutionary Biology, Zoological Institute, University of Basel, Vesalgasse 1
- Peter Ladurner
- Institute of Zoology and Center for Molecular Biosciences Innsbruck, University of Innsbruck, Technikerstr. 25
- Eugene Berezikov
- European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1
- DOI
- https://doi.org/10.1038/s41467-017-02214-8
- Journal volume & issue
-
Vol. 8,
no. 1
pp. 1 – 12
Abstract
Regeneration capable flatworms have emerged as powerful models for studying stem cell biology and patterning, however their study has been hindered by the lack of transgenesis methods. Here, the authors describe a transgenesis method for Macrostomum lignano, as well as a new annotated genome sequence.
