Diagnostic value of 8-isoprostane and transforming growth factor-β in bronchial asthma patients

Abstract

Abstract Background Asthma is an airway inflammatory disease with functional and structural changes, leading to bronchial hyperreponsiveness and airflow obstruction. 8-Isoprostane (8-iso-PGF2α) is considered a marker of oxidative stress specific to lipid peroxidation, transforming growth factor β1 (TGFβ1) as an important fibrogenic and immunomodulatory factor known to induce structural changes associated with asthma. Objective We aimed to study the diagnostic value of 8-iso-PGF2α and TGFβ1 in asthmatic patients. Patients and methods Samples of serum and bronchoalveolar lavage fluid from 40 asthmatic patients (20 moderate and 20 severe) and 10 healthy volunteers were assessed for their levels of 8-iso-PGF2α and TGFβ1. Results Bronchoalveolar lavage 8-iso-PGF2α and TGFβ1 was higher in asthmatic patients. It was significantly increased with increased asthma severity. Conclusion Increased levels of TGFβ1 and 8-iso-PGF2α is associated with disease severity. However, there is need for continued exploration on the mechanisms responsible for these structural changes.

Keywords

• airflow obstruction
• asthma
• biomarker
• hyperreponsiveness
• inflammation
• 8-iso-prostaglandin F2α