FISH-method: technique of cytogenetic retrospective dose evaluation

Abstract

The purpose of this work was to submit the review of data of the scientific literature devoted to a FISH method as a way of cytogenetic retrospective dose evaluation. Problems of cytogenetic retrospective assessment of a dose in general; molecular and biological bases of a FISH-method; types of chromosome aberrations identified by means of a FISH-method; the principles of creation of dose curves for the FISH-registered chromosome translocations have been considered. The conclusion is drawn on need of attraction of three-color and multi-color variants of this technique to retrospective dose evaluation.

Keywords

• chromosome aberrations
• dose evaluation
• FISH-method
• peripheral blood lymphocyte culture