The effect of germatranol in a liposomal emulsion on the processes of lipid peroxidation and the state of the antioxidant system of the rat body

Abstract

Summary. It was studied the effect of a 0.1 % solution of germatranol in a liposomal emulsion on the processes of lipid peroxidation and the state of the antioxidant system in the body of rats. It was established, that germanium in liposomal emulsion has a cytoprotective and antioxidant effect in the body of rats on immunocompetent cells under conditions of endotoxemia. The obtained data will be used in the design of a dosage form of germanium in a liposomal emulsion. The aim of the study – to learn the effect of germatranol in a liposomal emulsion on the processes of lipid peroxidation and the state of the antioxidant system of the rat body. Materials and Methods. The experiment was conducted on two groups of sexually mature non-linear white rats with body weight 120–130 g, aged 2.3–3.0 months, (5 animals in a group), with the same housing conditions. Animals of the control (first) group received a standard diet with feeding granulated compound feed throughout the entire period of the experiment with free access to water. The animals of the experimental (second) group were injected subcutaneously with a 0.1 % solution of germatranol in a liposomal emulsion, in a dose of 2.0 cm3, for four days in a row. The results of the research were processed statistically using the program Microsoft Excel 2003. The probability assessment was carried out according to the Student's criterion, and the results of average values were considered statistically probable р≤0.05; р≤0.01 and р≤0.001. Results. According to the results of the study, it was found that after the use of liposomal emulsion with germatranol, the content of lipid hydroperoxides in the body is likely to decrease with TBA-active products compared to the indicators of rats in the control group. Also, the antioxidant system is activation in the body of rats: the enzymatic activity of superoxide dismutase and glutathione peroxidase boosted the antioxidant system of the body of rats to combat oxidative stress and reduced the course of the inflammatory process in damaged cells. Taking into account the fact that catalase is localized in peroxisomes, germatranol in liposomal emulsion activated the reaction of splitting excess hydrogen peroxide in the body of rats. In this way, peroxide was utilized in mitochondria, microsomes, and peroxisomes. Conclusions. The use of 0.1 % germatranol solution in a liposomal emulsion to rats contributed to a probable reduction of TBA-active products by 18.0 % in their body compared to the indicators of the control group and the activity of the body's antioxidant protection, as evidenced by a probable increase in the activity of superoxide dismutase by 12.0 %, glutathione peroxidase by 30.0 %, and catalase by 22.0 %

Keywords

• germanium
• liposomes
• rats
• lipid peroxidation
• antioxidant system