The Effect of Cold Plasma on Selected Parameters of Bovine Colostrum

Abstract

The main problem in processing bovine colostrum is preserving as many beneficial compounds as possible, most of which have low thermal stability. The present study evaluates the possibility of using cold plasma (CP) as a decontamination technology and its effect on selected biologically active fractions of freeze-dried bovine colostrum. The plasma process was carried out in air, nitrogen, and oxygen environments. The results revealed that the sterilization process using CP caused slight changes in the colour of the samples expressed by the attributes ΔC, ΔL, Δh and ΔE. The decontamination effect depended on the gas used and the type of microorganism. The highest decontamination effects were gained under oxygen conditions, where reductions were obtained for total psychrophilic bacteria (THPC) by log 1.24, mesophilic bacteria (THMC) by log 1.02, Enterobacteriaceae by log 1.16, E. coli by log 0.96, yeast (TYMC) by log 0.92. A significantly lower decontaminating effect was obtained for Gram-positive bacteria and sporophytic forms. Additionally, the application of CP, regardless of the gas used, affected the modification of protein structure and reduction of immunoglobulin concentration. as proven by proteomics analyses (1-DE, 2-DE, MALDI–TOF MS). The same applied to β-lactoglobulin in air and oxygen and BSA in nitrogen and air.

Keywords

• cold plasma
• colostrum
• proteome
• decontamination technology
• microbiological safety