Life (Oct 2024)

Inverse Shifting-PCR Modified by Capillary Electrophoresis for Detecting F8 <i>int22h</i> and <i>int1h</i> Inversions in Severe Hemophilia A Patients and Probable Carriers

  • Rosa Michel Martínez-Contreras,
  • Silvia Sofía García-López,
  • Hilda Luna-Záizar,
  • Ana Rebeca Jaloma-Cruz

DOI
https://doi.org/10.3390/life14101332
Journal volume & issue
Vol. 14, no. 10
p. 1332

Abstract

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Globally, intron 22 inversions (Inv22s) of the factor VIII gene (F8) are the most frequent pathogenic variants and account for 45–50% of severe hemophilia A (SHA) cases, while intron 1 inversion (Inv1) explains 1–5% of SHA cases. The detection of both inversions by an inverse shifting-polymerase chain reaction (IS-PCR) is the first choice worldwide for the diagnosis of patients and carriers of SHA. To improve its sensitivity and reproducibility in the visualization of PCR products, we approached the IS-PCR with fluorescent capillary electrophoresis instead of agarose electrophoresis. Based on the original protocol, we modified two primers by 5’-end labeling with FAM™ fluorescent dye for the detection of the PCR products by capillary electrophoresis. Additionally, the “fast enzymes” BclI and T4-Ligase were incorporated for work saving in the genomic digestion and ligation reactions, respectively. Once we accomplished the standardization and verified the reproducibility of the modified IS-PCR method, we applied it for the diagnosis of a cohort of SHA patients and carriers. The modified IS-PCR by fluorescent capillary electrophoresis for PCR product detection is more sensitive than agarose electrophoresis. The method was also improved by using the new rapid enzymes to save time in the whole process.

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