Structural basis of ligand binding modes of human EAAT2

Zhenglai Zhang; Huiwen Chen; Ze Geng; Zhuoya Yu; Hang Li; Yanli Dong; Hongwei Zhang; Zhuo Huang; Juquan Jiang; Yan Zhao

doi:10.1038/s41467-022-31031-x

Nature Communications (Jun 2022)

Structural basis of ligand binding modes of human EAAT2

Zhenglai Zhang,
Huiwen Chen,
Ze Geng,
Zhuoya Yu,
Hang Li,
Yanli Dong,
Hongwei Zhang,
Zhuo Huang,
Juquan Jiang,
Yan Zhao

Affiliations

Zhenglai Zhang: Department of Microbiology and Biotechnology, College of Life Sciences, Northeast Agricultural University
Huiwen Chen: Department of Microbiology and Biotechnology, College of Life Sciences, Northeast Agricultural University
Ze Geng: State Key Laboratory of Natural and Biomimetic Drugs, Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Sciences, Peking University Health Science Center
Zhuoya Yu: National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
Hang Li: National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
Yanli Dong: National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
Hongwei Zhang: National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
Zhuo Huang: State Key Laboratory of Natural and Biomimetic Drugs, Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Sciences, Peking University Health Science Center
Juquan Jiang: Department of Microbiology and Biotechnology, College of Life Sciences, Northeast Agricultural University
Yan Zhao: National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences

DOI: https://doi.org/10.1038/s41467-022-31031-x
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 10

Abstract

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Excitatory amino acid transporter 2 (EAAT2) mediates glutamate uptake in the synapse. Here, authors report cryo-EM structures of the human EAAT2 in complexes with glutamate or selective inhibitor WAY-213613, revealing important insights into substrate recognition and selective inhibition.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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