Collateral Prospects of Polyherbal Formulation as Antidiabetic and Appraising its Toxicity in Early Development of Zebrafish (Danio Rerio) Larvae

Abstract

The polyherbal formulation was prepared using leaves, flowers, fruits of these specific plants Ficus religiosa, Allium sativum, Senna auriculata, Andrographis paniculata, Momordica charantia, Eugenia jambolana (FASAME). The hydroalcoholic extracts of the FASAME and its phytochemical constituents was determined. For toxicity study, the zebra fishes are used as the experimental models and were exposed to different concentration of FASAME formulation (100 μg, 70 μg, 50 μg, 10 μg, and 5 μg). Then its viability and its lethal effect was calculated. Finally the antidiabetic activity of FASAME was determined by β - galactosidase assay.The phytochemical constituents like flavonoids, steroids, terpenoid, saponins, resins, carbohydrates, proteins and essential oil was present in the hydroalcoholic extract of FASAME polyherbal formulation .The effect of FASAME formulation on Zebra fish larvae has a substantial increase in the percentage of viability at their lower concentration. The FASAME formulation exhibited selective anti-diabetic activity. These results showed that the FASAME formulation has anti-diabetic activity and can be used for the treatment of diabetics.The phytochemical analysis of FASAME drug has a mixture of phytochemical compounds and their IC50 value was found to be 74.39 μg/ml. The toxicity analysis on Zebra fish larvae also showed higher viability at their lower concentration and proved to be a powerful antidiabetic drug for diabetics.

Keywords

• : Anti-diabetic activity, Polyherbal formulation, phytochemical, FASAME formulation Zebra fish larvae.