Communications Biology (Oct 2022)
Honing-in antigen-specific cells during antibody discovery: a user-friendly process to mine a deeper repertoire
- Ankit Mahendra,
- Aftabul Haque,
- Ponraj Prabakaran,
- Brian C. Mackness,
- Thomas P. Fuller,
- Xiaohua Liu,
- Sagar V. Kathuria,
- Yui-Hsi Wang,
- Nilesh Amatya,
- Xiaocong Yu,
- Joern Hopke,
- Dietmar Hoffmann,
- Eva Bric-Furlong,
- Ningning Zhang,
- Hyun-Suk Cho,
- Ruijun Zhang,
- Jose Sancho,
- Jacqueline Saleh,
- Sambasiva P. Rao,
- Maria Wendt,
- Partha S. Chowdhury
Affiliations
- Ankit Mahendra
- Large Molecule Research, Sanofi
- Aftabul Haque
- Large Molecule Research, Sanofi
- Ponraj Prabakaran
- Large Molecule Research, Sanofi
- Brian C. Mackness
- Large Molecule Research, Sanofi
- Thomas P. Fuller
- Large Molecule Research, Sanofi
- Xiaohua Liu
- Large Molecule Research, Sanofi
- Sagar V. Kathuria
- Large Molecule Research, Sanofi
- Yui-Hsi Wang
- Immunology & Inflammation Research, Genomics Center, Sanofi
- Nilesh Amatya
- Immunology & Inflammation Research, Genomics Center, Sanofi
- Xiaocong Yu
- Large Molecule Research, Sanofi
- Joern Hopke
- Molecular Expression and Screening Technologies, Sanofi
- Dietmar Hoffmann
- Molecular Expression and Screening Technologies, Sanofi
- Eva Bric-Furlong
- Molecular Expression and Screening Technologies, Sanofi
- Ningning Zhang
- Large Molecule Research, Sanofi
- Hyun-Suk Cho
- Large Molecule Research, Sanofi
- Ruijun Zhang
- Large Molecule Research, Sanofi
- Jose Sancho
- Neuroinflammation, Sanofi Genzyme
- Jacqueline Saleh
- Neuroinflammation, Sanofi Genzyme
- Sambasiva P. Rao
- Large Molecule Research, Sanofi
- Maria Wendt
- Large Molecule Research, Sanofi
- Partha S. Chowdhury
- Large Molecule Research, Sanofi
- DOI
- https://doi.org/10.1038/s42003-022-04129-7
- Journal volume & issue
-
Vol. 5,
no. 1
pp. 1 – 17
Abstract
The authors developed a methodology for bulk enrichment of antigen-specific B cells to improve the diversity of antibody sequences and increase the number of functional antigen-specific antibodies coming from different antibody generation platforms.
