Cord Blood Plasma and Placental Mesenchymal Stem Cells-Derived Exosomes Increase Ex Vivo Expansion of Human Cord Blood Hematopoietic Stem Cells While Maintaining Their Stemness

Rasha S. Teleb; Amal Abdul-Hafez; Amira Othman; Ahmed El-Abd Ahmed; Abdelrahman A. Elsaid; Hattan Arif; Ahmed A. Zarea; Mohammed Abdulmageed; Hend Mohamed; Sherif Abdelfattah Ibrahim; Ranga P. Thiruvenkataramani; Tarek Mohamed; Masamitsu Kanada; Burra V. Madhukar; Myrna Gonzalez Arellano; Mohammed M. Sayed; Heba M. Qubaisy; Said A. Omar

doi:10.3390/cells12020250

Cells (Jan 2023)

Cord Blood Plasma and Placental Mesenchymal Stem Cells-Derived Exosomes Increase Ex Vivo Expansion of Human Cord Blood Hematopoietic Stem Cells While Maintaining Their Stemness

Rasha S. Teleb,
Amal Abdul-Hafez,
Amira Othman,
Ahmed El-Abd Ahmed,
Abdelrahman A. Elsaid,
Hattan Arif,
Ahmed A. Zarea,
Mohammed Abdulmageed,
Hend Mohamed,
Sherif Abdelfattah Ibrahim,
Ranga P. Thiruvenkataramani,
Tarek Mohamed,
Masamitsu Kanada,
Burra V. Madhukar,
Myrna Gonzalez Arellano,
Mohammed M. Sayed,
Heba M. Qubaisy,
Said A. Omar

Affiliations

Rasha S. Teleb: Division of Neonatology, Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA
Amal Abdul-Hafez: Division of Neonatology, Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA
Amira Othman: Division of Neonatology, Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA
Ahmed El-Abd Ahmed: Department of Pediatrics and Neonatology, Faculty of Medicine, South Valley University, Qena 83523, Egypt
Abdelrahman A. Elsaid: Clinical Pathology Department, Faculty of Medicine, South Valley University, Qena 83523, Egypt
Hattan Arif: Division of Neonatology, Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA
Ahmed A. Zarea: The Institute for Quantitative Health Science & Engineering, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA
Mohammed Abdulmageed: Division of Neonatology, Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA
Hend Mohamed: Division of Neonatology, Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA
Sherif Abdelfattah Ibrahim: Division of Neonatology, Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA
Ranga P. Thiruvenkataramani: Division of Neonatology, Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA
Tarek Mohamed: Division of Neonatology, Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA
Masamitsu Kanada: The Institute for Quantitative Health Science & Engineering, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA
Burra V. Madhukar: Division of Neonatology, Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA
Myrna Gonzalez Arellano: Division of Neonatology, Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA
Mohammed M. Sayed: Department of Pediatrics and Neonatology, Faculty of Medicine, South Valley University, Qena 83523, Egypt
Heba M. Qubaisy: Department of Pediatrics and Neonatology, Faculty of Medicine, South Valley University, Qena 83523, Egypt
Said A. Omar: Division of Neonatology, Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA

DOI: https://doi.org/10.3390/cells12020250
Journal volume & issue: Vol. 12, no. 2
p. 250

Abstract

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Background: Mesenchymal stem cells (MSCs) have been used for ex vivo expansion of umbilical cord blood (UCB) hematopoietic stem cells (HSCs) to maintain their primitive characters and long-term reconstitution abilities during transplantation. Therapeutic effects of MSCs mainly rely on paracrine mechanisms, including secretion of exosomes (Exos). The objective of this study was to examine the effect of cord blood plasma (CBP)-derived Exos (CBP Exos) and Placental MSCs-derived Exos (MSCs Exos) on the expansion of UCB HSCs to increase their numbers and keep their primitive characteristics. Methods: CD34+ cells were isolated from UCB, cultured for 10 days, and the expanded HSCs were sub-cultured in semisolid methylcellulose media for primitive colony forming units (CFUs) assay. MSCs were cultured from placental chorionic plates. Results: CBP Exos and MSCs Exos compared with the control group significantly increased the number of total nucleated cells (TNCs), invitro expansion of CD34+ cells, primitive subpopulations of CD34+38+ and CD34+38−Lin− cells (p p < 0.01). Conclusion: CBP- and placental-derived exosomes are associated with significant ex vivo expansion of UCB HSCs, while maintaining their primitive characters and may eliminate the need for transplantation of an additional unit of UCB.

Published in Cells

ISSN: 2073-4409 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General): Cytology
Website: https://www.mdpi.com/journal/cells

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