Chitosan– Gelatin (Ch-G) Thin Membrane with Bone Marrow Mesenchymal Stem Cells (BMSCs): A New Model As A Dressing Bandage

Abstract

Background and Purpose: Various methods for repairing defects and injuries such as wounds and cuts on the body have long been used. Biocompatible material into the body for the purposes of applying the Chitosan and gelatin are among them. On the other hand, recently the use and application of special cells in the bone marrow stromal stem cells (BMSCs) to produce a wide variety of growth factors and tropic factors in the healing and restoration of great concern was. Methods: In this study, a thin film of polymer solution (Ch-G) of mixture casting method, cells with BMSCs were obtained. For this purpose, cells were passage BMSCs after four steps, the thin membrane (Ch-G) were cultured. Adhesion of the BMSCs cells by inverted microscopy, the purity and the stem was examined by immunocytochemistry after the sixth day. Results: Demonstrate that the proper proliferation and apoptosis of cells during this period was low on the membrane. The results showed no significant difference between the cells on thin membranes (Ch-G) compared with the control group. Conclusion: Considering that BMSCs can easily be on thin-film cells (Ch-G), growth and reproduction are good, so it can cover the cell membrane as a dressing Adhesive Bandage to repair some defects, wounds used and bone fractures.

Keywords

• BMSCs
• Chitosan
• Gelatin
• Thin membrane