Monitoring G protein-coupled receptor and β-arrestin trafficking in live cells using enhanced bystander BRET

Yoon Namkung; Christian Le Gouill; Viktoria Lukashova; Hiroyuki Kobayashi; Mireille Hogue; Etienne Khoury; Mideum Song; Michel Bouvier; Stéphane A. Laporte

doi:10.1038/ncomms12178

Nature Communications (Jul 2016)

Monitoring G protein-coupled receptor and β-arrestin trafficking in live cells using enhanced bystander BRET

Yoon Namkung,
Christian Le Gouill,
Viktoria Lukashova,
Hiroyuki Kobayashi,
Mireille Hogue,
Etienne Khoury,
Mideum Song,
Michel Bouvier,
Stéphane A. Laporte

Affiliations

Yoon Namkung: Department of Medicine, Research Institute of the McGill University Health Center (RI-MUHC), McGill University
Christian Le Gouill: Department of Biochemistry and Institute for Research in Immunology and Cancer (IRIC), Université de Montréal
Viktoria Lukashova: Department of Biochemistry and Institute for Research in Immunology and Cancer (IRIC), Université de Montréal
Hiroyuki Kobayashi: Department of Biochemistry and Institute for Research in Immunology and Cancer (IRIC), Université de Montréal
Mireille Hogue: Department of Biochemistry and Institute for Research in Immunology and Cancer (IRIC), Université de Montréal
Etienne Khoury: Department of Medicine, Research Institute of the McGill University Health Center (RI-MUHC), McGill University
Mideum Song: Department of Medicine, Research Institute of the McGill University Health Center (RI-MUHC), McGill University
Michel Bouvier: Department of Biochemistry and Institute for Research in Immunology and Cancer (IRIC), Université de Montréal
Stéphane A. Laporte: Department of Medicine, Research Institute of the McGill University Health Center (RI-MUHC), McGill University

DOI: https://doi.org/10.1038/ncomms12178
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 12

Abstract

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Cellular signaling processes often involve trafficking of receptors and other proteins between subcellular compartments. Here the authors demonstrate a method based on the concept of Enhanced bystander Bioluminescence Resonance Energy Transfer (EbBRET) that allows efficient real time monitoring of endocytosis and trafficking.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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