Generation of Human iMelanocytes from Induced Pluripotent Stem Cells through a Suspension Culture System

Li-Ping Liu; Ning-Ning Guo; Yu-Mei Li; Yun-Wen Zheng

STAR Protocols (Jun 2020)

Generation of Human iMelanocytes from Induced Pluripotent Stem Cells through a Suspension Culture System

Li-Ping Liu,
Ning-Ning Guo,
Yu-Mei Li,
Yun-Wen Zheng

Affiliations

Li-Ping Liu: Institute of Regenerative Medicine, Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, Jiangsu 212001, China; Department of Dermatology, Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, Jiangsu 212001, China; University of Tsukuba Faculty of Medicine, Ibaraki 305-8575, Japan
Ning-Ning Guo: Institute of Regenerative Medicine, Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, Jiangsu 212001, China; Department of Dermatology, Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, Jiangsu 212001, China
Yu-Mei Li: Institute of Regenerative Medicine, Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, Jiangsu 212001, China; Department of Dermatology, Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, Jiangsu 212001, China; Corresponding author
Yun-Wen Zheng: Institute of Regenerative Medicine, Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, Jiangsu 212001, China; University of Tsukuba Faculty of Medicine, Ibaraki 305-8575, Japan; Yokohama City University School of Medicine, Yokohama 236-0004, Japan; Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan; Corresponding author

Journal volume & issue: Vol. 1, no. 1
p. 100004

Abstract

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Summary: Melanocytes, derived from neural crest cells, are involved in melanin production. This protocol describes a method to generate induced melanocytes (iMelanocytes) from human induced pluripotent stem cells (iPSCs) using a suspension culture system, which considerably improves the differentiation efficiency. The most critical parts of this protocol are the selection of a reliable iPSC line with strong potential to differentiate into melanocytes and their stemness maintenance.For complete information on the use and generation of this protocol, please refer to our Cell Reports article, Liu el al. (2019).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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