Protocol for assay of transposase accessible chromatin sequencing in non-model species

Abstract

Summary: The assay for transposase accessible chromatin (ATAC-seq) is a method for mapping genome-wide chromatin accessibility. Coupled with high-throughput sequencing, it enables integrative epigenomics analyses. ATAC-seq requires direct access to cell nuclei, a major challenge in non-model species such as small invertebrates, whose soft tissue is surrounded by a protective exoskeleton. Here, we present modifications of the ATAC-seq protocol for applications in small crustaceans, extending applications to non-model species.For complete information on the use and execution of this protocol, please refer to Buenrostro et al. (2013).

Keywords

• Bioinformatics
• Genomics
• Sequencing
• High-throughput screening