Single-cell multi-omics and lineage tracing to dissect cell fate decision-making

Abstract

Summary: The concept of cell fate relates to the future identity of a cell, and its daughters, which is obtained via cell differentiation and division. Understanding, predicting, and manipulating cell fate has been a long-sought goal of developmental and regenerative biology. Recent insights obtained from single-cell genomic and integrative lineage-tracing approaches have further aided to identify molecular features predictive of cell fate. In this perspective, we discuss these approaches with a focus on theoretical concepts and future directions of the field to dissect molecular mechanisms underlying cell fate.

Keywords

• cell fate decision
• mathematical and computational models
• multi-omics
• clonal tracking
• haematopoiesis
• embryogenesis