Isolation and characterization of Sprague-Dawley and Wistar Kyoto GFP rat embryonic stem cells

Giulia Coppiello; Gloria Abizanda; Natalia Aguado; Elena Iglesias; Olalla Iglesias-Garcia; Antonio Lo Nigro; Felipe Prosper; Xabier L. Aranguren

doi:10.1016/j.scr.2017.03.020

Stem Cell Research (May 2017)

Isolation and characterization of Sprague-Dawley and Wistar Kyoto GFP rat embryonic stem cells

Giulia Coppiello,
Gloria Abizanda,
Natalia Aguado,
Elena Iglesias,
Olalla Iglesias-Garcia,
Antonio Lo Nigro,
Felipe Prosper,
Xabier L. Aranguren

Affiliations

Giulia Coppiello: Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain
Gloria Abizanda: Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain
Natalia Aguado: Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain
Elena Iglesias: Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain
Olalla Iglesias-Garcia: Center of Regenerative Medicine in Barcelona (CMRB), Spain
Antonio Lo Nigro: Ri.Med Foundation, Department of Laboratory Medicine and Advanced Biotechnologies, IRCCS-ISMETT (Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione), Palermo, Italy
Felipe Prosper: Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain
Xabier L. Aranguren: Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain

DOI: https://doi.org/10.1016/j.scr.2017.03.020
Journal volume & issue: Vol. 21, no. C
pp. 40 – 43

Abstract

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We generated two rat embryonic stem cell (ESC) lines: ATCe-SD7.8 from Sprague-Dawley strain and ATCe-WK1 from Wistar Kyoto strain. Cells were marked with enhanced green fluorescent protein (eGFP) by transduction with a lentiviral vector. Cells present a normal karyotype and express pluripotency-associated markers. Pluripotency was tested in vivo with the teratoma formation assay. Cells maintain eGFP expression upon differentiation to the three-germ layers. These cells can be a useful tool for cell therapy studies and chimera generation as they can be easily tracked by eGFP expression.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal