Ultrafast coherent motion and helix rearrangement of homodimeric hemoglobin visualized with femtosecond X-ray solution scattering

Yunbeom Lee; Jong Goo Kim; Sang Jin Lee; Srinivasan Muniyappan; Tae Wu Kim; Hosung Ki; Hanui Kim; Junbeom Jo; So Ri Yun; Hyosub Lee; Kyung Won Lee; Seong Ok Kim; Marco Cammarata; Hyotcherl Ihee

doi:10.1038/s41467-021-23947-7

Nature Communications (Jun 2021)

Ultrafast coherent motion and helix rearrangement of homodimeric hemoglobin visualized with femtosecond X-ray solution scattering

Yunbeom Lee,
Jong Goo Kim,
Sang Jin Lee,
Srinivasan Muniyappan,
Tae Wu Kim,
Hosung Ki,
Hanui Kim,
Junbeom Jo,
So Ri Yun,
Hyosub Lee,
Kyung Won Lee,
Seong Ok Kim,
Marco Cammarata,
Hyotcherl Ihee

Affiliations

Yunbeom Lee: Department of Chemistry and KI for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST)
Jong Goo Kim: Department of Chemistry and KI for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST)
Sang Jin Lee: Department of Chemistry and KI for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST)
Srinivasan Muniyappan: Department of Chemistry and KI for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST)
Tae Wu Kim: Department of Chemistry and KI for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST)
Hosung Ki: Department of Chemistry and KI for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST)
Hanui Kim: Department of Chemistry and KI for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST)
Junbeom Jo: Department of Chemistry and KI for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST)
So Ri Yun: Department of Chemistry and KI for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST)
Hyosub Lee: Department of Chemistry and KI for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST)
Kyung Won Lee: Department of Chemistry and KI for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST)
Seong Ok Kim: Department of Chemistry and KI for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST)
Marco Cammarata: European Synchrotron Radiation Facility
Hyotcherl Ihee: Department of Chemistry and KI for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST)

DOI: https://doi.org/10.1038/s41467-021-23947-7
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 12

Abstract

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Femtosecond time-resolved X-ray solution scattering (fs-TRXSS) measurements provide information on the structural dynamics of proteins in solution. Here, the authors present a structure refinement method for the analysis of fs-TRXSS data and use it to characterise the ultrafast structural changes of homodimeric haemoglobin.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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