Microbial Biotechnology (Apr 2024)

Insights into the biosynthesis of palladium nanoparticles for oxygen reduction reaction by genetically engineered bacteria of Shewanella oneidensis MR‐1

  • Shihui Li,
  • Jingwen Huang,
  • Linjun Tong,
  • Qingxin Li,
  • Haikun Zhou,
  • Xiaoting Deng,
  • Jin Zhou,
  • Zhiyong Xie,
  • Xueduan Liu,
  • Yili Liang

DOI
https://doi.org/10.1111/1751-7915.14469
Journal volume & issue
Vol. 17, no. 4
pp. n/a – n/a

Abstract

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Abstract Owing to the increasing need for green synthesis and environmental protection, the utilization of biological organism‐derived carbons as supports for noble‐metal electrocatalysts has garnered public interest. Nevertheless, the mechanism by which microorganisms generate nanometals has not been fully understood yet. In the present study, we used genetically engineered bacteria of Shewanella oneidensis MR‐1 (∆SO4317, ∆SO4320, ∆SO0618 and ∆SO3745) to explore the effect of surface substances including biofilm‐associated protein (bpfA), protein secreted by type I secretion systems (TISS) and type II secretion systems (T2SS), and lipopolysaccharide in microbial synthesis of metal nanoparticles. Results showed Pd/∆SO4317 (the catalyst prepared with the mutant ∆SO4317) shows better performance than other biocatalysts and commercial Pd/C, where the mass activity (MA) and specific activity (SA) of Pd/∆SO4317 are 3.1 and 2.1 times higher than those of commercial Pd/C, reaching 257.49 A g−1 and 6.85 A m−2 respectively. It has been found that the exceptional performance is attributed to the smallest particle size and the presence of abundant functional groups. Additionally, the absence of biofilms has been identified as a crucial factor in the formation of high‐quality bio‐Pd. Because the absence of biofilm can minimize metal agglomeration, resulting in uniform particle size dispersion. These findings provide valuable mechanical insights into the generation of biogenic metal nanoparticles and show potential industrial and environmental applications, especially in accelerating oxygen reduction reactions.