iScience (Nov 2021)
Bacteroides spp. promotes branched-chain amino acid catabolism in brown fat and inhibits obesity
- Naofumi Yoshida,
- Tomoya Yamashita,
- Tatsunori Osone,
- Tetsuya Hosooka,
- Masakazu Shinohara,
- Seiichi Kitahama,
- Kengo Sasaki,
- Daisuke Sasaki,
- Takeshi Yoneshiro,
- Tomohiro Suzuki,
- Takuo Emoto,
- Yoshihiro Saito,
- Genki Ozawa,
- Yushi Hirota,
- Yasuyuki Kitaura,
- Yoshiharu Shimomura,
- Yuko Okamatsu-Ogura,
- Masayuki Saito,
- Akihiko Kondo,
- Shingo Kajimura,
- Takeshi Inagaki,
- Wataru Ogawa,
- Takuji Yamada,
- Ken-ichi Hirata
Affiliations
- Naofumi Yoshida
- Division of Cardiovascular Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 6500017, Japan
- Tomoya Yamashita
- Division of Cardiovascular Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 6500017, Japan; AMED-PRIME, Japan Agency for Medical Research and Development, 1-8-1 Inohana, Chuo-ku, Tokyo 1008152, Japan; Corresponding author
- Tatsunori Osone
- School and Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Tokyo 1528550, Japan
- Tetsuya Hosooka
- Laboratory of Nutritional Physiology, School of Food and Nutritional Sciences/Graduate Division of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka 4228526, Japan
- Masakazu Shinohara
- Division of Epidemiology, Kobe University Graduate School of Medicine, Kobe 6500017, Japan; The Integrated Center for Mass Spectrometry, Graduate School of Medicine, Kobe University, Kobe 6500017, Japan
- Seiichi Kitahama
- Department of Metabolic and Bariatric Surgery, Center for Obesity, Diabetes and Endocrinology, Chibune General Hospital, Osaka 5550034, Japan
- Kengo Sasaki
- Graduate School of Science, Technology and Innovation, Kobe University, Kobe 6578501, Japan; Bio Palette Co., Ltd., Kobe 6500047, Japan
- Daisuke Sasaki
- Graduate School of Science, Technology and Innovation, Kobe University, Kobe 6578501, Japan
- Takeshi Yoneshiro
- Division of Metabolic Medicine, Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo 1538904, Japan
- Tomohiro Suzuki
- Laboratory of Epigenetics and Metabolism, Institute for Molecular and Cellular Regulation, Gunma University, Gunma 3718512, Japan
- Takuo Emoto
- Division of Cardiovascular Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 6500017, Japan
- Yoshihiro Saito
- Division of Cardiovascular Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 6500017, Japan
- Genki Ozawa
- TechnoSuruga Laboratory Co., Ltd., Shizuoka 4240065, Japan
- Yushi Hirota
- Division of Diabetes and Endocrinology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Kobe 6500017, Japan
- Yasuyuki Kitaura
- Laboratory of Nutritional Biochemistry, Department of Applied Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 4648601, Japan
- Yoshiharu Shimomura
- Department of Food and Nutritional Sciences, College of Bioscience and Biotechnology, Chubu University, Kasugai, Aichi 4878501, Japan
- Yuko Okamatsu-Ogura
- Faculty of Veterinary Medicine, Hokkaido University, Sapporo 0600818, Japan
- Masayuki Saito
- Faculty of Veterinary Medicine, Hokkaido University, Sapporo 0600818, Japan
- Akihiko Kondo
- Graduate School of Science, Technology and Innovation, Kobe University, Kobe 6578501, Japan
- Shingo Kajimura
- Division of Endocrinology, Diabetes & Metabolism, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
- Takeshi Inagaki
- Laboratory of Epigenetics and Metabolism, Institute for Molecular and Cellular Regulation, Gunma University, Gunma 3718512, Japan
- Wataru Ogawa
- Division of Diabetes and Endocrinology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Kobe 6500017, Japan
- Takuji Yamada
- School and Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Tokyo 1528550, Japan
- Ken-ichi Hirata
- Division of Cardiovascular Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 6500017, Japan
- Journal volume & issue
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Vol. 24,
no. 11
p. 103342
Abstract
Summary: The gut microbiome has emerged as a key regulator of obesity; however, its role in brown adipose tissue (BAT) metabolism and association with obesity remain to be elucidated. We found that the levels of circulating branched-chain amino acids (BCAA) and their cognate α-ketoacids (BCKA) were significantly correlated with the body weight in humans and mice and that BCAA catabolic defects in BAT were associated with obesity in diet-induced obesity (DIO) mice. Pharmacological systemic enhancement of BCAA catabolic activity reduced plasma BCAA and BCKA levels and protected against obesity; these effects were reduced in BATectomized mice. DIO mice gavaged with Bacteroides dorei and Bacteroides vulgatus exhibited improved BAT BCAA catabolism and attenuated body weight gain, which were not observed in BATectomized DIO mice. Our data have highlighted a possible link between the gut microbiota and BAT BCAA catabolism and suggest that Bacteroides probiotics could be used for treating obesity.
