Gold Nanostructure Orchestrated Electrochemical Immunosensor Integrated with Antibody-Electroactive Probe Conjugate for Rapid Detection of SARS-CoV-2 Antibody

Abstract

Viral detection has been studied predominantly in the last few years, along with the morbidity of COVID-19. Biosensors have been widely used for the detection of various biological molecules, showing a high potential for miniaturization and a friendly approach towards detection. Nanomaterials play a significant role in the development of biosensing devices due to their distinct morphological, optical, electrical, chemical, and physical properties, which improve their sensing efficiency. Therefore, the present work reports the fabrication of an electrochemical immunosensor adorned with gold nanoparticles coupled to a redox indicator-labeled antibody conjugate for the rapid detection of SARS-CoV-2 antibodies. The fabricated immunosensor can detect SARS-CoV-2 antibodies within a linear detection range of 10–100 ngmL−1 and offer a sensitivity of 0.013 × 10−3 mA ng−1mLmm−2. The adopted concept can be extended further for the detection of other viral antibodies with high sensitivity and display high prospects for miniaturization, hence offering tremendous commercialization potential.

Keywords

• SARS-CoV-2
• electrochemical biosensor
• gold nanoparticles
• conjugate