Single-cell RNA cap and tail sequencing (scRCAT-seq) reveals subtype-specific isoforms differing in transcript demarcation

Abstract

Most single-cell RNA sequencing methods have limited ability to profile the transcriptome at isoform resolution. Here the authors develop scRCAT-seq to characterize the 5′- and 3′-ends of transcripts in single cells, and identify cell-type specific alternative transcript isoforms.