Acta Biomedica Scientifica (Sep 2012)

ISOLATION OF GLICOPROTEID FROM THE FIXED RABIES VIRUS, STRAIN «MOSCOW 3253», AND CONSTRUCTING OF DOT-IMMUNOASSAY DIAGNOSTICUM ON ITS BASIS

  • N. A. Sharapova,
  • M. N. Kireyev,
  • E. G. Abramova,
  • S. V. Generalov,
  • Zh. V. Matveyeva,
  • I. M. Zhulidov,
  • N. E. Tereshkina,
  • E. A. Mikheyeva,
  • L. V. Savitskaya,
  • A. V. Gayeva,
  • A. K. Nikiforov

Journal volume & issue
Vol. 0, no. 5(1)
pp. 347 – 350

Abstract

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Described here are the results of glicoproteid isolation from the fixed rabies virus, strain «Moscow 3253», using non-ionic detergent with subsequent chromatographic purification. The obtained antigen was demonstrated to be applicable as immunoreagent for construction of diagnosticum, by means of conjugation with colloid gold nanoparticles. The diagnosticum is meant for detection of specific antibodies in immune sera of horsesproducers, and in the preparation of anti-rabies immunoglobulin, in dot-immunoassay.

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