Revista da Sociedade Brasileira de Medicina Tropical ()

Prevalence, and virulence determination of Listeria monocytogenes strains isolated from clinical and non-clinical samples by multiplex polymerase chain reaction

  • Abazar Pournajaf,
  • Ramazan Rajabnia,
  • Mansour Sedighi,
  • Aziz Kassani,
  • Vahid Moqarabzadeh,
  • Lida Lotfollahi,
  • Abdollah Ardebilli,
  • Behzad Emadi,
  • Gholamreza Irajian

DOI
https://doi.org/10.1590/0037-8682-0403-2015
Journal volume & issue
Vol. 49, no. 5
pp. 624 – 627

Abstract

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Abstract INTRODUCTION: This study aimed to determine the prevalence, and virulence factors of Listeria monocytogenes isolated from various samples by multiplex polymerase chain reaction (MPCR). METHODS: A total of 617 isolates were obtained and MPCR was employed for detection of the inlA, inlC, and inlJ genes. RESULTS: L. monocytogenes was detected in 46 (7.45%) of the 617 specimens. inlA, inlC, and inlJ were detected in 100%, 76.26%, and 71% isolates, respectively. CONCLUSIONS: This study validated MPCR in the analysis and rapid detection of L. monocytogenes. The role of the genes in pathogenesis of the strains can also be affirmed.

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