Isolate the bacteria Pseudomonas aeruginosa from different clinical samples and study the effect of pyocyanin pigments of some pathogenic bacterial

Abstract

Three hundred clinical sample were collected from patients admitted and comers to (Azadi Teaching, Kirkuk General, kidsGeneral) hospitals, 140 sample from females with percentage (46.6%) and 160 sample frommale (53.3%), and through it found that 24 samples with percentage (17.14%) represented positive for bacterial growth in females and 26 samples with(16.25%) represented positive bacterial growth of male cases. The ability of Pseudo. aeruginosa to produce pyocyanin on various media were show that 43 clinical isolates of Pseudo.aeruginosa (86%) produced pyocyanin on two media (Nutrient agar, Muller hinton agar) and 15 (30%) produced the pigment on Macconky agar and 24 isolates (48%) on blood agar. While the effect of pyocyanin pigment on the growth of different pathogenic bacterial isolates the results showed that a gram positive bacteria such as Staphylococcus aureus was more sensitive to the pyocyanin pigment than gram negative bacteria (E.coli, klebsiella pneumonia) when grown on Nutrient agar or Muller hinton agar.

Keywords

• pseudomonas aeruginosa
• pyocyanin
• antibacteria