Neuropsychiatric Disease and Treatment (Sep 2020)

MicroRNA-382-5p Targets Nuclear Receptor Subfamily 3 Group C Member 1 to Regulate Depressive-Like Behaviors Induced by Chronic Unpredictable Mild Stress in Rats

  • Li S,
  • Ma H,
  • Yuan X,
  • Zhou X,
  • Wan Y,
  • Chen S

Journal volume & issue
Vol. Volume 16
pp. 2053 – 2061

Abstract

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Shuqian Li,1 Hong Ma,2 Xu Yuan,1 Xiaomei Zhou,1 Yiwen Wan,1 Shangjie Chen1 1Department of Rehabilitation, People’s Hospital of Shenzhen Baoan District, Shenzhen 518100, People’s Republic of China; 2Department of Rehabilitation, Binzhou Medical University, Yantai, Shandong Province 264003, People’s Republic of ChinaCorrespondence: Shangjie ChenDepartment of Rehabilitation, People’s Hospital of Shenzhen Baoan District, 118 Longjing Second Road, Xin’an Street, Shenzhen, Baoan District 518100, People’s Republic of ChinaEmail [email protected]: Depression is an emotional disorder characterized by depression, lack of pleasure, and cognitive and sleep disorders. It is a systemic disease with a complex pathogenesis. In this study, we will be focused to investigate their associations and the exact functional mechanisms of miR-382-5p and NR3C1 in depression.Materials and Methods: We measured the expressions of microRNA-382-5p (miR-382-5p) and NR3C1 in the hippocampus by chronic unpredictable mild stress (CUMS). Depression behavior test including novelty-suppressed feeding test (NSFT), sucrose preference test (SPT), and forced swim test (FST) on rats have been conducted to examine the roles and functions of miR-382-5p and NR3C1 on depression-like behaviors by lentivirus vectors.Results: Up-regulation of miR-382-5p and down-regulation of NR3C1 were observed in rats’ hippocampus induced by CUMS. miR-382-5p targeted NR3C1 and inhibited the expressions of NR3C1 in rats’ hippocampus. miR-382-5p could significantly change the depression behaviors induced by CUMS. NR3C1 downstream BDNF and p-TrkB were also oppositely associated with miR-382-5p in rats’ hippocampus.Conclusion: Through our experiments and analysis, we found that the associations between miR-382-5p and NR3C1 could affect the depression-like behaviors.Keywords: miRNA-382-5p, depression, hippocampus, NR3C1

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