A cell-free nanobody engineering platform rapidly generates SARS-CoV-2 neutralizing nanobodies

Xun Chen; Matteo Gentili; Nir Hacohen; Aviv Regev

doi:10.1038/s41467-021-25777-z

Nature Communications (Sep 2021)

A cell-free nanobody engineering platform rapidly generates SARS-CoV-2 neutralizing nanobodies

Xun Chen,
Matteo Gentili,
Nir Hacohen,
Aviv Regev

Affiliations

Xun Chen: Klarman Cell Observatory, Broad Institute of MIT and Harvard
Matteo Gentili: Broad Institute of MIT and Harvard
Nir Hacohen: Broad Institute of MIT and Harvard
Aviv Regev: Klarman Cell Observatory, Broad Institute of MIT and Harvard

DOI: https://doi.org/10.1038/s41467-021-25777-z
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 14

Abstract

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Faster, higher throughput antibody engineering methods are needed. Here the authors present CeVICA, a cell-free nanobody engineering platform using ribosome display and computational clustering analysis for in vitro selection; they use this to develop nanobodies against the RBD of SARS-CoV-2 spike protein.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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