A genetically encoded fluorescent biosensor for extracellular l-lactate

Yusuke Nasu; Ciaran Murphy-Royal; Yurong Wen; Jordan N. Haidey; Rosana S. Molina; Abhi Aggarwal; Shuce Zhang; Yuki Kamijo; Marie-Eve Paquet; Kaspar Podgorski; Mikhail Drobizhev; Jaideep S. Bains; M. Joanne Lemieux; Grant R. Gordon; Robert E. Campbell

doi:10.1038/s41467-021-27332-2

Nature Communications (Dec 2021)

A genetically encoded fluorescent biosensor for extracellular l-lactate

Yusuke Nasu,
Ciaran Murphy-Royal,
Yurong Wen,
Jordan N. Haidey,
Rosana S. Molina,
Abhi Aggarwal,
Shuce Zhang,
Yuki Kamijo,
Marie-Eve Paquet,
Kaspar Podgorski,
Mikhail Drobizhev,
Jaideep S. Bains,
M. Joanne Lemieux,
Grant R. Gordon,
Robert E. Campbell

Affiliations

Yusuke Nasu: Department of Chemistry, School of Science, The University of Tokyo, Bunkyo-ku
Ciaran Murphy-Royal: Hotchkiss Brain Institute, Cumming School of Medicine, Department of Physiology and Pharmacology, University of Calgary
Yurong Wen: Department of Biochemistry, University of Alberta
Jordan N. Haidey: Hotchkiss Brain Institute, Cumming School of Medicine, Department of Physiology and Pharmacology, University of Calgary
Rosana S. Molina: Department of Microbiology and Cell Biology, Montana State University
Abhi Aggarwal: Janelia Research Campus, Howard Hughes Medical Institute
Shuce Zhang: Department of Chemistry, University of Alberta
Yuki Kamijo: Department of Chemistry, School of Science, The University of Tokyo, Bunkyo-ku
Marie-Eve Paquet: CERVO Brain Research Center, Laval University
Kaspar Podgorski: Janelia Research Campus, Howard Hughes Medical Institute
Mikhail Drobizhev: Department of Microbiology and Cell Biology, Montana State University
Jaideep S. Bains: Hotchkiss Brain Institute, Cumming School of Medicine, Department of Physiology and Pharmacology, University of Calgary
M. Joanne Lemieux: Department of Biochemistry, University of Alberta
Grant R. Gordon: Hotchkiss Brain Institute, Cumming School of Medicine, Department of Physiology and Pharmacology, University of Calgary
Robert E. Campbell: Department of Chemistry, School of Science, The University of Tokyo, Bunkyo-ku

DOI: https://doi.org/10.1038/s41467-021-27332-2
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 12

Abstract

Read online

l-lactate is an important intercellular energy currency. Here the authors report a genetically encoded biosensor eLACCO1.1 to monitor extracellular l-lactate; they use eLACCO1.1 to image extracellular l-lactate in cultured mammalian cells and brain tissue.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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