“Evaluation of salivary levels of heat shock protein 70 using ELISA among nonsmokers, conventional smokers, and reverse smokers”: A prospective study

Abstract

Background: Heat shock response is coordinated by five families of proteins with varying molecular weights known as heat shock proteins (HSP100, 90, 70, 60 and small HSPs). Members of HSP70 family are expressed during tumor cell differentiation, elevated temperatures, necrosis, exposure to nicotine etc., and act as chaperones facilitating newly synthesized proteins through intracellular compartments. Reverse smoking, where intraoral temperature could is very deleterious, well documented to cause malignancies and other pathological change. This present study was conducted to analyze the salivary levels of HSP 70 in conventional, reverse and non-smokers by using ELISA. Materials and Methods: A total of 90 individuals were included in the study comprising of conventional smokers (30), Reverse smokers (30) and non-smokers (30). 2ml of saliva was collected from all the individuals and subjected to centrifuge at 3000 rpm for 20mins. The collected supernatant was subjected to ELISA. Results: Increased salivary levels of HSP70 were observed in controls followed by reverse and conventional smokers (p value 0.000). Post Hoc analysis of HSP70 among conventional and reverse smokers, conventional and non-smokers, reverse and non-smokers was statistically significant between the all groups. Conclusion: Increased salivary HSP70 levels in non-smokers could be because the control group is due to their higher metabolism and possible hormonal imbalance in younger individuals. Reverse smokers had higher levels of salivary HSP70 due to the increased intraoral temperature and oxidative stress caused by the smoke. Hence, Smoking in any form could significantly alter the salivary HSP70 levels.

Keywords

• conventional smokers
• elisa
• hsp70
• non-smokers
• reverse smokers