Nature Communications (Jun 2018)
Molecular basis of Tousled-Like Kinase 2 activation
- Gulnahar B. Mortuza,
- Dario Hermida,
- Anna-Kathrine Pedersen,
- Sandra Segura-Bayona,
- Blanca López-Méndez,
- Pilar Redondo,
- Patrick Rüther,
- Irina Pozdnyakova,
- Ana M. Garrote,
- Inés G. Muñoz,
- Marina Villamor-Payà,
- Cristina Jauset,
- Jesper V. Olsen,
- Travis H. Stracker,
- Guillermo Montoya
Affiliations
- Gulnahar B. Mortuza
- Structural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen
- Dario Hermida
- Structural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen
- Anna-Kathrine Pedersen
- Mass Spectrometry for Quantitative Proteomics, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen
- Sandra Segura-Bayona
- Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology (BIST)
- Blanca López-Méndez
- Protein Production and Characterization Platform, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen
- Pilar Redondo
- Macromolecular Crystallography Group, Structural Biology and Biocomputing Program, Spanish National Cancer Research Center (CNIO)
- Patrick Rüther
- Mass Spectrometry for Quantitative Proteomics, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen
- Irina Pozdnyakova
- Protein Production and Characterization Platform, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen
- Ana M. Garrote
- Macromolecular Crystallography Group, Structural Biology and Biocomputing Program, Spanish National Cancer Research Center (CNIO)
- Inés G. Muñoz
- Macromolecular Crystallography Group, Structural Biology and Biocomputing Program, Spanish National Cancer Research Center (CNIO)
- Marina Villamor-Payà
- Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology (BIST)
- Cristina Jauset
- Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology (BIST)
- Jesper V. Olsen
- Mass Spectrometry for Quantitative Proteomics, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen
- Travis H. Stracker
- Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology (BIST)
- Guillermo Montoya
- Structural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen
- DOI
- https://doi.org/10.1038/s41467-018-04941-y
- Journal volume & issue
-
Vol. 9,
no. 1
pp. 1 – 17
Abstract
The Tousled-like kinase (TLKs) family belongs to a distinct branch of Ser/Thr kinases that exhibit the highest levels of activity during DNA replication. Here the authors present the crystal structure of the kinase domain from human TLK2 and propose an activation model for TLK2 based on biochemical and phosphoproteomics experiments.
