Nature Communications (Aug 2019)

3D sub-diffraction imaging in a conventional confocal configuration by exploiting super-linear emitters

  • Denitza Denkova,
  • Martin Ploschner,
  • Minakshi Das,
  • Lindsay M. Parker,
  • Xianlin Zheng,
  • Yiqing Lu,
  • Antony Orth,
  • Nicolle H. Packer,
  • James A. Piper

DOI
https://doi.org/10.1038/s41467-019-11603-0
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 12

Abstract

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Super-resolution microscopy is a valuable tool in bioimaging, but often requires complex systems or post-processing. Here, the authors present super-linear excitation-emission (SEE) microscopy, which overcomes these limitations by taking advantage of markers with super-linear dependence between emission and excitation power.