Novel phytosterol with Antimicrobial Potential of Digera muricata Mart. from Chittorgarh Region, India

Abstract

Digera muricata Mart, a plant having therapeutic characteristics that has been utilised traditionally, belongs to the Amaranthaceae family, and a promising source of specific natural products utilized as antioxidant, prophylactic, antimicrobial, anthelmintic, anti-diabetic, and allelopathic agent. In the present study, a biologically active phytosterol was isolated from Digera muricata Mart. The isolated compound was characterized by 13C, 1H NMR, FTIR, and HRMS. Characterization of the isolate was done by antimicrobial assay, and molecular docking. The antimicrobial potential of the isolated phytosterol (50 µl) against Streptococcus pyogenes was found to be maximum (ZOI-20.0 ± 1.0), followed by Streptococcus agalactiae (ZOI-11.3±1.5), Candida albicans (ZOI- 09.0 ± 1.0), Klebsiella pneumonia (ZOI-8.6 ± 1.5) and Escherichia coli (ZOI-8.6 ± 1.5). The molecular docking results indicate that the phytosterol binds to the receptor 1AI9 at the 32th and 58th positions; 1KZN receptor at the 76th position, the 5L3J receptor at the 46th (ASN) and 136th (ARG) position; 7WIJ receptor at the 419th (ARG) and 582th (ASP) and 585th (ASN) positions.

Keywords

• antimicrobial
• antifungal
• phytosterol
• digera muricata
• molecular docking