Serological and molecular detection of Leptospira spp in dogs

Abstract

Abstract INTRODUCTION: This study aimed to detect anti-Leptospira spp antibodies and Leptospira DNA in domestic dogs. METHODS: Blood and urine from 106 dogs were evaluated by microscopic agglutination test (MAT) and polymerase chain reaction (PCR), respectively. RESULTS: Six (5.7%) and one (1%) animals were positive by MAT and PCR, respectively. CONCLUSIONS: These results show a low prevalence of infection by Leptospira spp. The absence of positive results for the Icterohaemorrhagiae serogroup indicates the small relevance of these dogs as sources of human leptospirosis.

Keywords

• Leptospirosis
• PCR
• Microscopic
• Agglutination test