Química Nova (Jan 2013)

Metabolic response induced by endophytic fungi and bacteria in H. marrubioides Epling in vitro microplants

  • Luciana Cristina Vitorino,
  • Fabiano Guimarães Silva,
  • William Cardoso Lima,
  • Marcos Antônio Soares,
  • Rita Cássia Nascimento Pedroso,
  • Marolí Rodrigues Silva,
  • Herbert Júnior Dias,
  • Antônio Eduardo Miller Crotti,
  • Márcio Luís Andrade e Silva,
  • Wilson Roberto Cunha,
  • Patrícia Mendonça Pauletti,
  • Ana Helena Januário

DOI
https://doi.org/10.1590/S0100-40422013000700016
Journal volume & issue
Vol. 36, no. 7
pp. 1014 – 1020

Abstract

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Hyptis marrubioides Epling is a native plant from Brazilian Cerrado. In this paper, the response of in vitro microplants of this species to inoculation with bacterial and fungal endophytic isolates is evaluated. HPLC-DAD analysis showed the presence of 3,4-O-(Z)-dicaffeoylquinic acid and quercetin-7-O-glucoside as the main components. GC/MS analysis demonstrated that the sesquiterpenes τ-cadinol and caryophyllene oxide were only produced in microplants inoculated with endophytic bacteria, while methyl hexadecanoate, methyl heptadecanoate and methyl (Z,Z,Z) 9,12,15-octadecatrienoate and the triterpene methyl 3β-hydroxy-urs-12-en-28-oate were overexpressed only when the microplant was treated with endophytic fungi.

Keywords