Response surface methodology for optimizing the glycerolysis reaction of olive oil by Candida rugosa lipase

Abstract

In the present work, solvent free olive oil glycerolysis for the monoglycerides (MG) and diglycerides (DG) production with an immobilized Candida rugosa lipase was studied. MG and DG production were optimized using experiment design techniques and response surface methodology (RSM). RSM based on five-level, a five-variable central composite design (CCD) was used to optimize MG and DG production: reaction time, temperature, molar ratio of glycerol to oil, amount of lipase, and water content in glycerol. The reaction time, temperature, and amount of lipase were observed to be the most significant factors on the process response. The immobilized Candida rugosa lipase revealed optimum yield of MG and DG as 38.71 and 40.45 wt% respectively following a 5h reaction time with 0.025 g of lipase and 5% water content in glycerol at 40°C temperature. The yield of MG and DG production can be enhanced 1.5 fold by RSM.

Keywords

• monoglycerides
• diglycerides
• immobilized Candida rugosa lipase
• response surface methodology
• modeling