Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain

Myungjae Song; Jung Min Lim; Seonwoo Min; Jeong-Seok Oh; Dong Young Kim; Jae-Sung Woo; Hiroshi Nishimasu; Sung-Rae Cho; Sungroh Yoon; Hyongbum Henry Kim

doi:10.1038/s41467-021-25928-2

Nature Communications (Sep 2021)

Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain

Myungjae Song,
Jung Min Lim,
Seonwoo Min,
Jeong-Seok Oh,
Dong Young Kim,
Jae-Sung Woo,
Hiroshi Nishimasu,
Sung-Rae Cho,
Sungroh Yoon,
Hyongbum Henry Kim

Affiliations

Myungjae Song: Department of Pharmacology, Yonsei University College of Medicine
Jung Min Lim: Department of Pharmacology, Yonsei University College of Medicine
Seonwoo Min: Department of Electrical and Computer Engineering, Seoul National University
Jeong-Seok Oh: Department of Life Sciences, Korea University
Dong Young Kim: Department of Pharmacology, Yonsei University College of Medicine
Jae-Sung Woo: Department of Life Sciences, Korea University
Hiroshi Nishimasu: Structural Biology Division, Research Center for Advanced Science and Technology, The University of Tokyo
Sung-Rae Cho: Graduate School of Medical Science, Brain Korea 21 Plus Project for Medical Sciences, Yonsei University College of Medicine
Sungroh Yoon: Department of Electrical and Computer Engineering, Seoul National University
Hyongbum Henry Kim: Department of Pharmacology, Yonsei University College of Medicine

DOI: https://doi.org/10.1038/s41467-021-25928-2
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 8

Abstract

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While prime editing is a promising technology, PE2 systems often have low efficiency. Here the authors fuse a Rad51 DNA-binding domain to create hyPE2 with improved editing efficiency.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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